구강편평세포암종 세포주에서 유로키나제형 플라스미노젠 활성제 및 제일형 플라스미노젠 활성억제제 발현
Urokinase Type Plasminogen Activator and Plasminogen Aativator Inhibitor-1 Expression In Oral Squamous Cell Carcinoma Cell Line
유연상, 천윤권, 박경주, 이종헌,
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유연상 ( Yu Yeon-Sang ) - 단국대학교 치과대학 구강병리학교실
천윤권 ( Chun Yoon-Kwon ) - 단국대학교 치과대학 구강병리학교실
박경주 ( Park Gyeong-Ju ) - 단국대학교 치과대학 구강병리학교실
이종헌 ( Lee Chong-Heon ) - 단국대학교 치과대학 구강병리학교실
KMID : 0829220100340060331
Abstract
Tumor cell biological factors, such as urokinase plasminogen activator(uPA) and its inhibitor plasminogen activator inhibitor-1(PAI-1) play a role in tumor invasion, metastasis, and proliferation. These factors in patients with primary oral squamous cell carcinoma(Oral SCC) will be evaluated and correlated with clinicopathologic variables. However, relatively rarely has been known in oral squamous cell carcinoma in vivo and in vitro study. The purpose of this study were to investigate the protein expression of uPA and PAI-1 in oral SCC cell lines cell line compared to NHOK and to study migration and adhesion assay. All the cell lines were cultured under KBM bullet kit at 37℃ in a 5% CO2 incubator. We studied a possible association between cytosolic uPA and PA-1 concentrations in oral SCC cell line compared to NHOK using an enzyme-linked immunoassay(ELISA). Cell adhesion and migration assay were done in all the cell lines. In migration assay oral SCC cell lines were about 70 folds higher than NHOK. In adhesion assay oral SCC cell line were about 7-12 folds higher than NHOK. uPA cytosolic concentrations was about 15-19 folds and PAI-1 was 3 to 4.5 folds than that of NHOK. Both uPA and PAI-1 concentrations were correlated with migration and adhesion assay. High cytosolic concentrations of uPA and PAI-1 were correlated with migration and adhesion assay. It suggested that these markers might be specific for oral SCC cell line and these results would be contributed to treatment and prognosis of human oral squamous cell carcinoma.
키워드
UPA; PAI-1 Expression; Oral SCC Cell Line
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